Quality Control of Mitochondria in Parkinson's disease (PD) Using ATP and Bradford Assays
- OLCIAS
- May 1, 2023
- 2 min read
Title Talk: Quality Control of Mitochondria in Parkinson's Disease (PD) Using ATP and Bradford Assays
Author:
Al-Baraa Akram El-Sayed
De montfort University in Leicester
United kingdom
Email ID:
albraaakram94@gmail.com
Volume: Parkinson's Disease 2023_CN37_AA01
Abstract
Parkinson's disease is a heterogeneous, multifactorial and often complex disease characterized by motor impairment due to the presence of Lewy bodies and prominent degeneration of dopaminergic neurons in the substantia nigra.
Although the specific pathogenesis involving PD remains under investigation, mitochondrial dysfunction has been widely accepted as one of the major pathogenic pathways underlying the development of PD. Based on the hypothesis that depiction of HtrA2 (serine protease gene, mitochondrial precursor) might contribute to an increase in mitochondrial stress and transcriptional up regulation of the nuclear stress response CHOP gene. The present study aimed to analyze through laboratory based research the role of HtrA2 and CHOP in the transmission of stress signaling and the consequent activation of mitochondrial quality control in Parkinson's disease using ATP and Bradford assays.
96-well plates of Bradford assay Biography: Al-Baraa Akram is a clinical pharmacist and assistant lecturer in De montfort University in Leicester-United kingdom in faculty of health and life sciences- department of pharmaceutical biotechnology. He had his bachelor degree in clinical pharmacy from faculty of pharmacy-Assiut University in Egypt and he had his master degree in pharmaceutical biotechnology from De montfort University in Leicester in United kingdom. He published 9 journal articles and 2 books, he has also 7 patents. He works also as a pharmacist in Egypt for more than 5 years. He participated in many international conferences as a lecturer and worked as a demonstrator in faculty of pharmacy-pharmacognosy department in 6th October University in Egypt and awarded the best scientific research in Arab republic of Egypt in 2017 from Aswan University and he awarded scholarships in Russia, United Kingdom and Germany. Recent publications:
1-Al-Baraa Akram, Glenn McCann (2021) Effect of antimicrobial peptides and chemicals produced by animals on Aspergillus fumigatus, Advances in Bioscience and Biotechnology, 2021, 12, 173-192
2-Al-Baraa Akram, Glenn McCann (2021) Effect of antimicrobial peptides and chemicals produced by animals on liver microsomal enzymes CYP450
3- Al-Baraa Akram, (2021), getting fuel from sucrose (table sugar), journal of sustainable bioenergy Systems, 2021, 11, 272-284
4-Al-Baraa Akram, Glenn McCann (2022) Identification transgenic DNA of transformed Arabidopsis using PCR and Southern blot, Advances in Bioscience and Biotechnology, 2022
5-Al-Baraa Akram (2022) Quality control of mitochondria in Parkinson's disease (PD) using ATP and Bradford assays, Journal of neurological disorders vol 10:3, 2022
6-Akram A, McCann G. Effect of Antimicrobial peptides and chemicals produced by animals on Streptococcus pyogenes. Biomed Transl Sci. 2021; 1(3):1-9.
